Title |
Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts
|
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Published in |
Frontiers in Plant Science, June 2015
|
DOI | 10.3389/fpls.2015.00433 |
Pubmed ID | |
Authors |
Sona Fekecsová, Maksym Danchenko, Lubica Uvackova, Ludovit Skultety, Martin Hajduch |
Abstract |
The aim of the work was to test a relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7 cm immobilized pH gradient strips for the determination of clinically relevant proteins in wheat grain. Using this approach, 157 2-DE spots were quantified in biological triplicate, out of which 55 were identified by matrix-assisted laser desorption/ionization - time of flight tandem mass spectrometry. Clinically relevant proteins associated with celiac disease, wheat dependent exercise induced anaphylaxis, baker's asthma, and food allergy, were detected in 24 2-DE spots. However, alcohol-soluble gliadins were not detected with this approach. The comparison with a recent quantitative study suggested that gel-based and gel-free proteomics approaches are complementary for the detection and quantification of clinically relevant proteins in wheat grain. |
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Mendeley readers
Geographical breakdown
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 22% |
Student > Bachelor | 4 | 15% |
Student > Master | 3 | 11% |
Researcher | 3 | 11% |
Professor > Associate Professor | 2 | 7% |
Other | 3 | 11% |
Unknown | 6 | 22% |
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Engineering | 2 | 7% |
Computer Science | 2 | 7% |
Unspecified | 1 | 4% |
Other | 3 | 11% |
Unknown | 9 | 33% |