Title |
Effects of Substance P and Substance K on the Growth of Cultured Keratinocytes
|
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Published in |
Journal of Investigative Dermatology, March 1988
|
DOI | 10.1111/1523-1747.ep12456487 |
Pubmed ID | |
Authors |
Toshihiro. Tanaka, Kiichiro. Danno, Kouichi. Ikai, Sadao. Imamura |
Abstract |
The effects of substance P and substance K, which are coexpressed in the same mRNA as a beta-preprotachykinin in peripheral tissues and released in the inflammatory lesion of the skin, were examined on epidermal proliferation using spontaneously transformed mouse epidermal cell line (Pam 212 cells). Substance P stimulated the synthesis of DNA of Pam 212 cells in the medium containing 2%-10% fetal calf serum (FCS). Stimulation of DNA synthesis was dose dependent if the cells were cultured in the medium containing 2% FCS (quiescent condition). This effect was inhibited by spantide. In a serum-free medium, substance P had no effect on keratinocyte proliferation. In contrast, substance K, which shares a common amino acid sequence with substance P on its C-terminal, did not affect DNA synthesis of Pam 212 cells in either medium condition. Substance P released in inflammation may stimulate epidermal proliferation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 25% |
Student > Ph. D. Student | 4 | 25% |
Student > Postgraduate | 2 | 13% |
Professor > Associate Professor | 2 | 13% |
Other | 1 | 6% |
Other | 1 | 6% |
Unknown | 2 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 38% |
Medicine and Dentistry | 4 | 25% |
Neuroscience | 2 | 13% |
Unknown | 4 | 25% |