Title |
Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus
|
---|---|
Published in |
Frontiers in Pharmacology, June 2018
|
DOI | 10.3389/fphar.2018.00619 |
Pubmed ID | |
Authors |
Xin Hua, Qin Yang, Wanjiang Zhang, Zhimin Dong, Shenye Yu, Stefan Schwarz, Siguo Liu |
Abstract |
This study aimed at investigating the antibacterial activity of aspidinol, an extract from Dryopteris fragrans (L.) Schott, against methicillin-resistant Staphylococcus aureus (MRSA). MRSA isolates were treated with aspidinol to determine the differential expression of genes and associated pathways following the drug treatment. Aspidinol displayed significant anti-MRSA activity, both in vivo (minimum inhibitory concentration = 2 μg/mL) and in vitro, and achieved an antibacterial effect comparable to that of vancomycin. In the lethal septicemic mouse study, a dose of 50 mg/kg of either aspidinol or vancomycin provided significant protection from mortality. In the non-lethal septicemic mouse study, aspidinol and vancomycin produced a significant reduction in mean bacterial load in murine organs, including the spleen, lung, and liver. After treatment with aspidinol, we found through RNA-seq and RT-PCR experiments that the inhibition of the formation of ribosomes was the primary S. aureus cell-killing mechanism, and the inhibition of amino acid synthesis and the reduction of virulence factors might play a secondary role. |
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Canada | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 51 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 12% |
Student > Master | 6 | 12% |
Student > Ph. D. Student | 3 | 6% |
Student > Bachelor | 3 | 6% |
Student > Doctoral Student | 2 | 4% |
Other | 5 | 10% |
Unknown | 26 | 51% |
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Biochemistry, Genetics and Molecular Biology | 4 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 8% |
Agricultural and Biological Sciences | 4 | 8% |
Chemistry | 2 | 4% |
Other | 4 | 8% |
Unknown | 27 | 53% |