Title |
Plasma and urine metabolite profiling reveals the protective effect of Clinacanthus nutans in an ovalbumin-induced anaphylaxis model: 1H-NMR metabolomics approach
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Published in |
Journal of Pharmaceutical & Biomedical Analysis, June 2018
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DOI | 10.1016/j.jpba.2018.06.038 |
Pubmed ID | |
Authors |
Leng Wei Khoo, Siew Foong Audrey Kow, M. Maulidiani, Ming Tatt Lee, Chin Ping Tan, Khozirah Shaari, Chau Ling Tham, Faridah Abas |
Abstract |
The present study sought to identify the key biomarkers and pathways involved in the induction of allergic sensitization to ovalbumin and to elucidate the potential anti-anaphylaxis property of Clinacanthus nutans (Burm. f.) Lindau water leaf extract, a Southeast Asia herb in an in vivo ovalbumin-induced active systemic anaphylaxis model evaluated by 1H-NMR metabolomics. The results revealed that carbohydrate metabolism (glucose, myo-inositol, galactarate) and lipid metabolism (glycerol, choline, sn-glycero-3-phosphocholine) are the key requisites for the induction of anaphylaxis reaction. Sensitized rats treated with 2000 mg/kg bw C. nutans extract before ovalbumin challenge showed a positive correlation with the normal group and was negatively related to the induced group. Further 1H-NMR analysis in complement with Kyoto Encyclopedia of Genes and Genomes (KEGG) reveals the protective effect of C. nutans extract against ovalbumin-induced anaphylaxis through the down-regulation of lipid metabolism (choline, sn-glycero-3-phosphocholine), carbohydrate and signal transduction system (glucose, myo-inositol, galactarate) and up-regulation of citrate cycle intermediates (citrate, 2-oxoglutarate, succinate), propanoate metabolism (1,2-propanediol), amino acid metabolism (betaine, N,N-dimethylglycine, methylguanidine, valine) and nucleotide metabolism (malonate, allantoin). In summary, this study reports for the first time, C. nutans water extract is a potential anti-anaphylactic agent and 1H-NMR metabolomics is a great alternative analytical tool to explicate the mechanism of action of anaphylaxis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 60 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 18% |
Student > Master | 8 | 13% |
Other | 6 | 10% |
Student > Bachelor | 6 | 10% |
Lecturer | 3 | 5% |
Other | 11 | 18% |
Unknown | 15 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 13% |
Chemistry | 7 | 12% |
Agricultural and Biological Sciences | 7 | 12% |
Medicine and Dentistry | 3 | 5% |
Other | 7 | 12% |
Unknown | 19 | 32% |