Title |
Streptococcal Endo-β-N-Acetylglucosaminidase Suppresses Antibody-Mediated Inflammation In Vivo
|
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Published in |
Frontiers in immunology, July 2018
|
DOI | 10.3389/fimmu.2018.01623 |
Pubmed ID | |
Authors |
Kutty Selva Nandakumar, Mattias Collin, Kaisa E. Happonen, Susanna L. Lundström, Allyson M. Croxford, Bingze Xu, Roman A. Zubarev, Merrill J. Rowley, Anna M. Blom, Christian Kjellman, Rikard Holmdahl |
Abstract |
Endo-β-N-acetylglucosaminidase (EndoS) is a family 18 glycosyl hydrolase secreted by Streptococcus pyogenes. Recombinant EndoS hydrolyzes the β-1,4-di-N-acetylchitobiose core of the N-linked complex type glycan on the asparagine 297 of the γ-chains of IgG. Here, we report that EndoS and IgG hydrolyzed by EndoS induced suppression of local immune complex (IC)-mediated arthritis. A small amount (1 µg given i.v. to a mouse) of EndoS was sufficient to inhibit IgG-mediated arthritis in mice. The presence of EndoS disturbed larger IC lattice formation both in vitro and in vivo, as visualized with anti-C3b staining. Neither complement binding in vitro nor antigen-antibody binding per se were affected. Thus, EndoS could potentially be used for treating patients with IC-mediated pathology. |
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