Title |
CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
|
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Published in |
Frontiers in Genetics, July 2018
|
DOI | 10.3389/fgene.2018.00240 |
Pubmed ID | |
Authors |
Bhaskar Roy, Jing Zhao, Chao Yang, Wen Luo, Teng Xiong, Yong Li, Xiaodong Fang, Guanjun Gao, Chabungbam O. Singh, Lise Madsen, Yong Zhou, Karsten Kristiansen |
Abstract |
Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and Cascade 9 (also known as Cas9, CRISPR associated protein 9) confer protection against invading viruses or plasmids. The CRISPR/Cascade 9 system constitutes one of the most powerful genome technologies available to researchers today. So far, this technology has enabled efficient genome editing and modification in several model organisms and has successfully been used in biomedicine and biomedical engineering. However, challenges for efficient and safe genetic manipulation in several organisms persist. Here, we review functional approaches and future challenges associated with the use of the CRISPR/Cascade 9 genome editing system and discuss opportunities, ethical issues and future directions within this field. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Switzerland | 2 | 18% |
United Kingdom | 1 | 9% |
Spain | 1 | 9% |
United States | 1 | 9% |
Unknown | 6 | 55% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 55% |
Scientists | 5 | 45% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 108 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 21 | 19% |
Student > Ph. D. Student | 15 | 14% |
Researcher | 14 | 13% |
Student > Master | 12 | 11% |
Professor | 6 | 6% |
Other | 13 | 12% |
Unknown | 27 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 36 | 33% |
Agricultural and Biological Sciences | 20 | 19% |
Medicine and Dentistry | 6 | 6% |
Social Sciences | 3 | 3% |
Computer Science | 2 | 2% |
Other | 11 | 10% |
Unknown | 30 | 28% |