Chapter title |
Illuminating Phytophthora Biology with Fluorescent Protein Tags
|
---|---|
Chapter number | 10 |
Book title |
Plant Pathogenic Fungi and Oomycetes
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8724-5_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8723-8, 978-1-4939-8724-5
|
Authors |
Audrey M. V. Ah-Fong, Meenakshi Kagda, Howard S. Judelson, Ah-Fong, Audrey M. V., Kagda, Meenakshi, Judelson, Howard S. |
Abstract |
Phytophthora species cause diseases that threaten agricultural, ornamental, and forest plants worldwide. Explorations of the biology of these pathogens have been aided by the availability of genome sequences, but much work remains to decipher the roles of their proteins. Insight into protein function can be obtained by visualizing them within cells, which has been facilitated by recent improvements in fluorescent protein and microscope technologies. Here, we describe strategies to permit investigators to generate strains of Phytophthora that express fluorescently tagged proteins and study their localization during growth in artificial media and during plant infection. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 20% |
Researcher | 1 | 20% |
Unknown | 3 | 60% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 40% |
Unknown | 3 | 60% |