Chapter title |
Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries
|
---|---|
Chapter number | 8 |
Book title |
The Ubiquitin Proteasome System
|
Published in |
Methods in molecular biology, September 2018
|
DOI | 10.1007/978-1-4939-8706-1_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8705-4, 978-1-4939-8706-1
|
Authors |
Wei Zhang, Sachdev S. Sidhu, Zhang, Wei, Sidhu, Sachdev S. |
Abstract |
Ubiquitination is a posttranslational protein modification pathway regulating diverse cellular processes that are implicated in numerous human diseases. However, targeting the enzymes in the ubiquitination cascade potently and selectively remains a major challenge. Recently we devised a methodology to generate ubiquitin-based modulators for E3 ligases and deubiquitinases, enzymes that control the specificity of protein ubiquitination and deubiquitination, respectively. Here, we describe methods to generate libraries of ubiquitin variants and perform phage display selections to isolate high-affinity binders for target proteins. Importantly, the strategy introduced here can be applied to other small protein domains mediating protein-protein interactions to engineer tools for target validation and potential therapeutic development. |
Mendeley readers
Geographical breakdown
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 29% |
Student > Bachelor | 4 | 19% |
Researcher | 3 | 14% |
Student > Master | 2 | 10% |
Other | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 19% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
Other | 0 | 0% |
Unknown | 5 | 24% |