Title |
Expression and clinical significance of Nectin-4 in hepatocellular carcinoma
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Published in |
OncoTargets and therapy, January 2016
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DOI | 10.2147/ott.s96999 |
Pubmed ID | |
Authors |
Jie Ma, ZhiYong Sheng, Yang Lv, WenBin Liu, QiYang Yao, TingTing Pan, ZhiJun Xu, ChuanHai Zhang, GeLiang Xu |
Abstract |
Nectin-4 is a member of the Nectin family of four Ca(+)-independent immunoglobulin-like cell adhesion molecules and implicated in cell adhesion, movement, proliferation, differentiation, polarization, and survival. The aberrant expression of Nectin-4 has been found in a variety of tumors; however, its expression in hepatocellular carcinoma (HCC) is still poorly understood. This study was to investigate the expression of Nectin-4 and its clinical significance in the patients with HCC. The expression of Nectin-4 was assessed at mRNA and protein levels by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting assays in 20 HCC specimens and adjacent non-tumor live tissues. Furthermore, the clinical significance of Nectin-4 in 87 cases of HCC was confirmed by immunohistochemistry. The mRNA and protein levels of Nectin-4 were higher in HCC tumor tissues than in the matched non-tumor tissues. Nectin-4 was located in the cytoplasm of tumor cells and over-expressed in 67.82% (59/87) HCC tissues by immunohistochemical staining. Positive Nectin-4 expression was significantly correlated with tumor size (P=0.029), status of metastasis (P=0.023), vascular invasion (P=0.018) and tumor-node-metastasis stage (P=0.003). In addition, Kaplan-Meier survival analysis indicated that positive Nectin-4 expression was associated with worse recurrence-free survival (RFS) and overall survival (OS) (P=0.006 and P=0.005, respectively). In multivariate analysis, Nectin-4 was an independent prognostic factor for RFS and OS in the patients with HCC. Nectin-4 is upregulated in HCC and may be a novel prognostic biomarker for the patients after surgical resection. |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 2 | 11% |
Student > Bachelor | 2 | 11% |
Unspecified | 1 | 5% |
Librarian | 1 | 5% |
Other | 2 | 11% |
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Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
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Unknown | 8 | 42% |