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Mendeley readers
| Chapter title |
Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models.
|
|---|---|
| Chapter number | 11 |
| Book title |
Tumor Immunology
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3338-9_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3336-5, 978-1-4939-3338-9
|
| Authors |
Benedetta Nicolis di Robilant, Maddalena Noviello, B. Nicolis di Robilant, di Robilant, B. Nicolis, Noviello, Maddalena |
| Editors |
Attilio Bondanza, Monica Casucci |
| Abstract |
RNA interference (RNAi) is a powerful tool for efficient and highly specific gene silencing. Transduction with lentiviral vectors provides stable and long-term gene expression in slowly and non-dividing cells. This chapter describes how to couple these two technologies to efficiently silence specific genes in primary acute myeloid leukemia (AML) cells for subsequent xenotransplantation in immunocompromised mice. This approach could be used for loss-of-function studies aimed at identifying oncogenic targets in AML. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 50% |
| Lecturer | 1 | 25% |
| Student > Master | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 50% |
| Nursing and Health Professions | 1 | 25% |
| Medicine and Dentistry | 1 | 25% |