Title |
A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
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Published in |
Frontiers in Plant Science, April 2016
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DOI | 10.3389/fpls.2016.00437 |
Pubmed ID | |
Authors |
Wen-Ying Zhu, Long Huang, Long Chen, Jian-Tao Yang, Jia-Ni Wu, Mei-Ling Qu, Dan-Qing Yao, Chun-Li Guo, Hong-Li Lian, Huan-Le He, Jun-Song Pan, Run Cai |
Abstract |
High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected. |
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