| Chapter title |
Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.
|
|---|---|
| Chapter number | 7 |
| Book title |
Dendritic Cell Protocols
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3606-9_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3604-5, 978-1-4939-3606-9
|
| Authors |
Charlotte L. Scott, Pamela B. Wright, Simon W. F. Milling, Allan McI Mowat, Scott, Charlotte L., Wright, Pamela B., Milling, Simon W. F., Mowat, Allan McI |
| Editors |
Elodie Segura, Nobuyuki Onai |
| Abstract |
The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 27% |
| Student > Master | 5 | 23% |
| Researcher | 2 | 9% |
| Professor | 2 | 9% |
| Other | 1 | 5% |
| Other | 2 | 9% |
| Unknown | 4 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 10 | 45% |
| Agricultural and Biological Sciences | 4 | 18% |
| Arts and Humanities | 1 | 5% |
| Economics, Econometrics and Finance | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 5 | 23% |