Title |
Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
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Published in |
Frontiers in Microbiology, May 2016
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DOI | 10.3389/fmicb.2016.00817 |
Pubmed ID | |
Authors |
Ahmed K. Al Atya, Yanath Belguesmia, Gabrielle Chataigne, Rozenn Ravallec, Anne Vachée, Sabine Szunerits, Rabah Boukherroub, Djamel Drider |
Abstract |
Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown in vitro by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices. |
X Demographics
Geographical breakdown
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Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 50% |
Scientists | 2 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 63 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 8 | 13% |
Student > Ph. D. Student | 7 | 11% |
Student > Master | 6 | 10% |
Student > Bachelor | 5 | 8% |
Professor > Associate Professor | 4 | 6% |
Other | 10 | 16% |
Unknown | 23 | 37% |
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Agricultural and Biological Sciences | 8 | 13% |
Immunology and Microbiology | 7 | 11% |
Medicine and Dentistry | 3 | 5% |
Chemistry | 2 | 3% |
Other | 6 | 10% |
Unknown | 26 | 41% |