Title |
Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize
|
---|---|
Published in |
Frontiers in Plant Science, March 2016
|
DOI | 10.3389/fpls.2016.00414 |
Pubmed ID | |
Authors |
Timothy Kelliher, Dakota Starr, Wenling Wang, Jamie McCuiston, Heng Zhong, Michael L. Nuccio, Barry Martin |
Abstract |
Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after independently controlling for inducer parent sex and transgene zygosity. CENH3 fusion proteins were localized to centromeres and did not cause vegetative defects or male sterility. CENH3:RNAi lines did not demonstrate consistent knockdown and rarely produced haploids. In contrast, many of the complemented CENH3 (-∕-) lines produced haploids at low frequencies. The rate of gynogenic haploid induction reached a maximum of 3.6% in several hemizygous individuals when backcrossed as males. These results demonstrate that CENH3-tailswap transgenes can be used to engineer in vivo haploid induction systems into maize plants. |
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