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X Demographics
Mendeley readers
Attention Score in Context
| Title |
D-galactan II is an immunodominant antigen in O1 lipopolysaccharide and affects virulence in Klebsiella pneumoniae: implication in vaccine design
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|---|---|
| Published in |
Frontiers in Microbiology, November 2014
|
| DOI | 10.3389/fmicb.2014.00608 |
| Pubmed ID | |
| Authors |
Pei-Fang Hsieh, Meng-Chuan Wu, Feng-Ling Yang, Chun-Tang Chen, Tzu-Chi Lou, Yi-Yin Chen, Shih-Hsiung Wu, Jin-Chuan Sheu, Jin-Town Wang |
| Abstract |
In the O1 strain of Klebsiella, the lipopolysaccharide (LPS) O-antigen is composed of D-galactan I and D-galactan II. Although the composition of the O1 antigen of Klebsiella was resolved more than two decades, the genetic locus involved in the biosynthesis of D-galactan II and the role of D-galactan II in bacterial pathogenesis remain unclear. Here, we report the identification of the D-galactan II-synthesizing genes by screening a transposon mutant library of an acapsulated Klebsiella pneumoniae O1 strain with bacteriophage. K. pneumoniae strain deleted for wbbY exhibited abrogated D-galactan II production; altered serum resistance and attenuation of virulence. Serologic analysis of K. pneumoniae clinical isolates demonstrated that D-galactan II was more prevalent in community-acquired pyogenic liver abscess (PLA)-causing strains than in non-tissue-invasive strains. WbbY homologs, WbbZ homologs, and lipopolysaccharide structures based on D-galactan II also were present in several Gram-negative bacteria. Immunization of mice with the magA-mutant (K(-) 1 O1) (that is, with a LPS D-galactan II-producing strain) provided protection against infection with an O1:K2 PLA strain. Our findings indicate that both WbbY and WbbZ homologs are sufficient for the synthesis of D-galactan II. D-galactan II represents an immunodominant antigen; is conserved among multiple species of Gram-negative bacteria and could be a useful vaccine candidate. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 20% |
| Student > Ph. D. Student | 7 | 18% |
| Researcher | 7 | 18% |
| Student > Master | 5 | 13% |
| Student > Doctoral Student | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 7 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 9 | 23% |
| Agricultural and Biological Sciences | 8 | 20% |
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Unspecified | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 4 | 10% |
| Unknown | 8 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 June 2023.
All research outputs
#4,448,251
of 24,129,125 outputs
Outputs from Frontiers in Microbiology
#4,466
of 27,172 outputs
Outputs of similar age
#60,796
of 371,258 outputs
Outputs of similar age from Frontiers in Microbiology
#34
of 190 outputs
Altmetric has tracked 24,129,125 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 27,172 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 371,258 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 190 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.