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The Influence of Microwave Sterilization on the Ultrastructure, Permeability of Cell Membrane and Expression of Proteins of Bacillus Cereus

Overview of attention for article published in Frontiers in Microbiology, September 2018
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Title
The Influence of Microwave Sterilization on the Ultrastructure, Permeability of Cell Membrane and Expression of Proteins of Bacillus Cereus
Published in
Frontiers in Microbiology, September 2018
DOI 10.3389/fmicb.2018.01870
Pubmed ID
Authors

Jin-Xuan Cao, Fang Wang, Xuan Li, Yang-Ying Sun, Ying Wang, Chang-Rong Ou, Xing-Feng Shao, Dao-Dong Pan, Dao-Ying Wang

Abstract

Bacillus cereus was isolated from ready-to-serve brine goose, identified by 16S rRNA gene sequencing analysis and treated with a commercial microwave sterilization condition (a power of 1,800 W at 85°C for 5 min). The influence of microwaves on the morphology, the permeability of membrane and the expression of total bacterial proteins was observed. Microwave induced the clean of bacterial nuclear chromatin, increased the permeability and disrupted the integrity of membrane. Twenty-three proteins including 18 expressed down-regulated proteins and 5 expressed up-regulated proteins were identified by HPLC-MS/MS in the samples treated with microwave. The frequencies of proteins changed after microwaves treatment were labeled as 39.13% (synthesis and metabolism of amino acid or proteins), 21.74% (carbohydrate metabolism), 8.70% (anti-oxidant and acetyl Co-A synthesis), and 4.35% (the catalyst of catabolism of bacterial acetoin, ethanol metabolism, glyoxylate pathway, butyrate synthesis and detoxification activity), respectively. This study indicates that microwaves result in the inactivation of Bacillus cereus by cleaning nuclear chromatin, disrupting cell membrane and disordering the expression of proteins.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 17%
Student > Master 3 13%
Student > Bachelor 2 8%
Student > Postgraduate 1 4%
Lecturer 1 4%
Other 1 4%
Unknown 12 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 13%
Engineering 2 8%
Environmental Science 1 4%
Biochemistry, Genetics and Molecular Biology 1 4%
Immunology and Microbiology 1 4%
Other 3 13%
Unknown 13 54%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2024.
All research outputs
#16,621,302
of 25,243,120 outputs
Outputs from Frontiers in Microbiology
#16,294
of 28,973 outputs
Outputs of similar age
#208,171
of 341,496 outputs
Outputs of similar age from Frontiers in Microbiology
#426
of 695 outputs
Altmetric has tracked 25,243,120 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 28,973 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,496 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 695 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.