Joseph V. Raimondo, Bradley Joyce, Louise Kay, Theresa Schlagheck, Sarah E. Newey, Shankar Srinivas, Colin J. Akerman
Abstract
Within the nervous system, intracellular Cl(-) and pH regulate fundamental processes including cell proliferation, metabolism, synaptic transmission, and network excitability. Cl(-) and pH are often co-regulated, and network activity results in the movement of both Cl(-) and H(+). Tools to accurately measure these ions are crucial for understanding their role under physiological and pathological conditions. Although genetically-encoded Cl(-) and pH sensors have been described previously, these either lack ion specificity or are unsuitable for neuronal use. Here we present ClopHensorN-a new genetically-encoded ratiometric Cl(-) and pH sensor that is optimized for the nervous system. We demonstrate the ability of ClopHensorN to dissociate and simultaneously quantify Cl(-) and H(+) concentrations under a variety of conditions. In addition, we establish the sensor's utility by characterizing activity-dependent ion dynamics in hippocampal neurons.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2017.
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#6,127,359
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Outputs from Frontiers in Cellular Neuroscience
#1,117
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#65,291
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Outputs of similar age from Frontiers in Cellular Neuroscience
#43
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Altmetric has tracked 22,729,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 4,215 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has gotten more attention than average, scoring higher than 73% of its peers.
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We're also able to compare this research output to 203 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.
