Zaijie Chen, Qianqian Cheng, Chanquan Hu, Xinrui Guo, Ziqiang Chen, Yan Lin, Taijiao Hu, Maria Bellizzi, Guodong Lu, Guo-Liang Wang, Zonghua Wang, Songbiao Chen, Feng Wang
Abstract
Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited a wide use of the XVE system in this important food crop. Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/loxP-FRT system, and optimized a seed-soaking procedure for XVE induction in rice. By using a gus gene and an hpRNAi cassette targeted for OsPDS as reporters, we demonstrated that soaking transgenic seeds with estradiol solution could induce highly efficient site-specific recombination in germinating embryos, resulting in constitutive and high-level expression of target gene or RNAi cassette in intact rice plants from induced seeds. The strategy reported here thereby provides a useful gene activation approach for effectively regulating gene expression in rice.
Pharmacology, Toxicology and Pharmaceutical Science
1
3%
Social Sciences
1
3%
Other
2
6%
Unknown
8
26%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2017.
All research outputs
#17,916,739
of 23,003,906 outputs
Outputs from Frontiers in Plant Science
#12,199
of 20,501 outputs
Outputs of similar age
#227,735
of 317,613 outputs
Outputs of similar age from Frontiers in Plant Science
#330
of 493 outputs
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