Title |
Analysis of CRISPR-Cas System in Streptococcus thermophilus and Its Application
|
---|---|
Published in |
Frontiers in Microbiology, February 2018
|
DOI | 10.3389/fmicb.2018.00257 |
Pubmed ID | |
Authors |
Mengyuan Hao, Yanhua Cui, Xiaojun Qu |
Abstract |
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) loci, which provide a specific immunity against exogenous elements, are hypervariable among distinct prokaryotes. Based on previous researches, this review focuses on concluding systematical genome editing protocols inStreptococcus thermophilus.Firstly, its protocols and optimized conditions in gene editing are introduced. What's more, classification and diversity analyses ofS. thermophilusCRISPR-Cas benefit the further understanding of evolution relationship amongStreptococcus.Ability of its foreign segment integration and spacer source analyses also indicate a new direction of phage resistance. Above all, all of these point out its potential to be regarded as another model system other than type II CRISPR-Cas inStreptococcus pyogenes. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 97 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 25 | 26% |
Student > Master | 14 | 14% |
Researcher | 9 | 9% |
Student > Ph. D. Student | 7 | 7% |
Student > Doctoral Student | 3 | 3% |
Other | 9 | 9% |
Unknown | 30 | 31% |
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Agricultural and Biological Sciences | 14 | 14% |
Immunology and Microbiology | 7 | 7% |
Unspecified | 3 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
Other | 8 | 8% |
Unknown | 35 | 36% |